It was first predicted in 1988 that there may be an Open Reading Frame (ORF) on the negative strand of the Human Immunodeficiency Virus type 1 (HIV-1) genome that could encode a protein named AntiSense Protein (ASP). In spite of some controversy, reports began to emerge some years later describing the detection of HIV-1 antisense transcripts, the presence of ASP in transfected and infected
1. One of the reasons why DNA is double stranded--this means DNA has sense and antisense strands--is to make a copy. During transcription, RNAs are transcribed referring to anti-sense strand sequences--making base pairs with anti-sense strand. In other words, the sense strand does not do anything during transcription.
Jorgensen RA, Cluster PD, English J, Que Q, Napoli CA. Chalcone synthase cosuppression phenotypes in petunia flowers: Comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences. Plant Mol Biol. 1996;31:957–973. DOI: 10.1007/BF00040715; 6. Cogoni C, Macino G. Post-transcriptional gene silencing across kingdoms.
There is therefore no biologically meaningful sense/antisense concept for entire genomes, chromosomes or plasmids. Read more in the Wikipedia article on sense, and the answers to this SE Biology question. Having said that, it should be clear that the expression cassettes pictured in figues A) and B) are completely identical. If you ignore the
The antisense strand usually serves the strand for synthesis of mRNA, therefore they are called coding strands. Reverse Primer. Reverse Primers are DNA stretches that bind to the sense strand (+) of the DNA that runs in the 5’ to 3’ direction. They amplify the strand they bind to. Forward vs Reverse Primer
Evolution of the genetic code: the nonsense, antisense, and antinonsense codes make no sense. 1999 Dec;54 (1-2):39-46. doi: 10.1016/s0303-2647 (99)00056-8. According to the molecular recognition theory, the complementarity of the sense and nonsense DNA strands is reflected in a complementarity of polypeptides and the corresponding nonsense
An entire DNA strand (going the length of a whole molecule) is not all "sense" or "antisense." "Watson" may be sense in one section and "Crick" may be sense in the other (as in the picture on handout 12-B). The terms "sense" and "transcribed" strand are defined for each section of the DNA that is transcribed as a unit (usually a gene or small
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dna sense vs antisense
